Ethical and technical considerations for the creation of cell lines in the head & neck and tissue harvesting for research and drug development (Part I): Techniques of tissue harvesting and propagation

doi:10.1186/1755-7682-2-8

International Archives of Medicine

Volume 2

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Upile T
Jerjes W
Kafas P
Singh SU
Sudhoff H
Mahil J
Sandison A
Hopper C

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Upile T
Jerjes W
Kafas P
Singh SU
Sudhoff H
Mahil J
Sandison A
Hopper C
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Review

Ethical and technical considerations for the creation of cell lines in the head & neck and tissue harvesting for research and drug development (Part I): Techniques of tissue harvesting and propagation

Tahwinder Upile¹^,2^,3^,7 , Waseem Jerjes¹^,3 , Panagiotis Kafas⁴ , Sandeep U Singh¹ , Holger Sudhoff⁵ , Jaspal Mahil¹ , Ann Sandison⁶ and Colin Hopper¹^,3

¹Head & Neck Centre, University College London Hospital, London, UK

²Head and Neck Department, Charing Cross Hospital, London, UK

³Department of Surgery, University College London Medical School, London, UK

⁴Department of Oral Surgery and Radiology, School of Dentistry, Aristotle University, Thessaloniki, Greece

⁵Department of Otolaryngology, Bielefeld University, Bielefeld, Germany

⁶Department of Histopathology, Imperial College & Charing Cross Hospital, London, UK

⁷The Royal National Throat, Nose and Ear Hospital, 330/332 Grays' Inn Road, London, WC1X 8EE, UK

author email corresponding author email

International Archives of Medicine 2009, 2:8doi:10.1186/1755-7682-2-8


Published:	3 April 2009

Abstract

Background

Although much has been published for the development of cell lines, these were lab based and developed for scientific technical staff.

Objective of review

We present a simple and successful protocol for the development of cell lines and tissue harvesting for the clinical scientist. We also discuss the ethical implications of tissue retention and present a generic consent form.

Conclusion

The advantages of hospital-based cell line creation are numerous. We can be more certain that cell lines are developed from the particular tissues of interest and accurate anatomical and appropriate clinico-pathological control tissues are also harvested. We can also be certain of less cell line cross contamination.