Review
Ethical and technical considerations for the creation of cell lines in the head & neck and tissue harvesting for research and drug development (Part I): Techniques of tissue harvesting and propagation
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* Corresponding author: Tahwinder Upile mrtupile@yahoo.com
1 Head & Neck Centre, University College London Hospital, London, UK
2 Head and Neck Department, Charing Cross Hospital, London, UK
3 Department of Surgery, University College London Medical School, London, UK
4 Department of Oral Surgery and Radiology, School of Dentistry, Aristotle University, Thessaloniki, Greece
5 Department of Otolaryngology, Bielefeld University, Bielefeld, Germany
6 Department of Histopathology, Imperial College & Charing Cross Hospital, London, UK
7 The Royal National Throat, Nose and Ear Hospital, 330/332 Grays' Inn Road, London, WC1X 8EE, UK
International Archives of Medicine 2009, 2:8 doi:10.1186/1755-7682-2-8
Published: 3 April 2009Abstract
Background
Although much has been published for the development of cell lines, these were lab based and developed for scientific technical staff.
Objective of review
We present a simple and successful protocol for the development of cell lines and tissue harvesting for the clinical scientist. We also discuss the ethical implications of tissue retention and present a generic consent form.
Conclusion
The advantages of hospital-based cell line creation are numerous. We can be more certain that cell lines are developed from the particular tissues of interest and accurate anatomical and appropriate clinico-pathological control tissues are also harvested. We can also be certain of less cell line cross contamination.