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Open Access Short report

Lack of reliability of nanotechnology in the of free plasma DNA in samples of patients with prostate cancer

Ricardo Moreno1, Pamela O Delgado1, Patrícia G Coelho1, Sarah R Marsicano1, Viviane AV Boas1, Ligia A Azzalis3, Virgínia BC Junqueira3, Katya C Rocha1, Luiz Carlos de Abreu3, Vitor E Valenti34, Jefferson Drezzet3, Edimar Cristiano Pereira2 and Fernando LA Fonseca12*

Author Affiliations

1 Laboratório de Análises Clínicas, Faculdade de Medicina do ABC, Av. Príncipe de Gales 821, Santo André, CEP: 09060-650, Brazil

2 Departamento de Ciências Biológicas, Universidade Federal de São Paulo, UNIFESP, Rua Prof. Artur Riedel, 275, Diadema, São Paulo 09972-270, Brazil

3 Laboratório de Delineamento de Estudos e Escrita Científica, Departamento de Morfologia e Fisiologia, Faculdade de Medicina do ABC, Santo André, Av. Príncipe de Gales, n. 821, Santo André, CEP: 09060-650, Brazil

4 Programa de Pós-Graduação em Fisioterapia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, UNESP, Rua Roberto Simonsen, 305, Presidente Prudente, São Paulo 19060-900, Brazil

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International Archives of Medicine 2013, 6:2  doi:10.1186/1755-7682-6-2

Published: 12 January 2013

Abstract

Background

Several studies seek biological markers that give diagnostic and degree of tumor development. The aim of this study was to validate the determination of plasma DNA using nanotechnology (Nanovue™-NV) in samples of 80 patients with prostate cancer.

Methods

Blood samples of 80 patients of the Urology Ambulatory of Faculdade de Medicina do ABC with prostate cancer confirmed by anatomical-pathology criteria were analyzed. DNA extraction was performed using a GFX TM kit (Amersham Pharmacia Biotech, Inc, USA) following the adapted protocol. Plasma was subjected to centrifugation.

Results

There was a big difference between the first and the second value obtained by NanoVue Only two samples had no differences between duplicates. Maximum difference between duplicates was 38 μg/mL. Average variation between 51 samples was 10.29 μg/mL, although 21 samples had differences above this average. No correlation was observed between pDNA obtained by traditional spectrophotometry and by nanotechnology.

Conclusion

Determination of plasma DNA by nanotechnology was not reproducible.

Keywords:
Nanotechnology; DNA; Neoplams; Prostate